pEGFP-hsRPA14 (MSW#503)
(Plasmid
#208069)
-
PurposeExpression of GFP-tagged hsRPA3 in human cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 208069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRPA3
-
Alt nameRPA14
-
SpeciesH. sapiens (human)
-
Entrez GeneRPA3 (a.k.a. REPA3, RP-A p14)
-
Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid to express GFP-hsRPA14 in human cells
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-hsRPA14 (MSW#503) was a gift from Marc Wold (Addgene plasmid # 208069 ; http://n2t.net/addgene:208069 ; RRID:Addgene_208069) -
For your References section:
Cellular functions of human RPA1. Multiple roles of domains in replication, repair, and checkpoints. Haring SJ, Mason AC, Binz SK, Wold MS. J Biol Chem. 2008 Jul 4;283(27):19095-111. doi: 10.1074/jbc.M800881200. Epub 2008 May 9. 10.1074/jbc.M800881200 PubMed 18469000