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pEGFP-hsRPA32 (MSW#497)
(Plasmid #208068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA2
  • Alt name
    RPA32
  • Species
    H. sapiens (human)
  • Mutation
    T88I- please see depositor comments
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid to express GFP-hsRPA32 in human cells. Please note there is a single mutation T88I in hsRPA32. Depositor confirms this mutation does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hsRPA32 (MSW#497) was a gift from Marc Wold (Addgene plasmid # 208068 ; http://n2t.net/addgene:208068 ; RRID:Addgene_208068)

  • For your References section:

    Cellular functions of human RPA1. Multiple roles of domains in replication, repair, and checkpoints. Haring SJ, Mason AC, Binz SK, Wold MS. J Biol Chem. 2008 Jul 4;283(27):19095-111. doi: 10.1074/jbc.M800881200. Epub 2008 May 9. 10.1074/jbc.M800881200 PubMed 18469000
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