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pEGFP-hsRPA70 (MSW#473)
(Plasmid #208067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA1
  • Alt name
    RPA70
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid to express GFP-hsRPA70 in human cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hsRPA70 (MSW#473) was a gift from Marc Wold (Addgene plasmid # 208067 ; http://n2t.net/addgene:208067 ; RRID:Addgene_208067)

  • For your References section:

    Cellular functions of human RPA1. Multiple roles of domains in replication, repair, and checkpoints. Haring SJ, Mason AC, Binz SK, Wold MS. J Biol Chem. 2008 Jul 4;283(27):19095-111. doi: 10.1074/jbc.M800881200. Epub 2008 May 9. 10.1074/jbc.M800881200 PubMed 18469000
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