p11d-tRPA (MSW#111)
(Plasmid #208066)

• Purpose: expression of human Replication Protein A in E. coli
• Depositing Lab: Marc Wold
• Publication: Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-32.

Backbone

• Vector backbone: pET11d
• Backbone manufacturer: MilliporeSigma
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPA1, RPA2, RPA3
• Alt name: RPA70, RPA32, RPA14
• Species: H. sapiens (human)
• Mutation: RPA32 has a point mutation Thr88(ACC) -> Ile(ATC)
• Entrez Gene: RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
• Entrez Gene: RPA3 (a.k.a. REPA3, RP-A p14)
• Entrez Gene: RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 111_ p11d-tRPA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a single T7 RNA polymerase promoter followed by RPA70, RPA14 and RPA32. (Each gene has Shine-Delgarno ribosome binding site.) Induction with IPTGexpresses RPA70, RPA14 and RPA32 which form reconbinant RPA complex.