OR51-consensus
(Plasmid #208065)

• Purpose: Consensus OR51 engineered from human OR51 family members
• Depositing Lab: Hiroaki Matsunami
• Publication: Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 10.1073/pnas.1915520117. Epub 2020 Jan 23.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4006
• Total vector size (bp): 5023
• Modifications to backbone: Rhodopsin tag at N terminus
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OR51-consensus
• Alt name: consOR51
• Species: Synthetic
• Insert Size (bp): 1017
• Promoter: CMV
• Tag / Fusion Protein:
  • Rhodopsin tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CTCCACAGGTGTCCACTC
• 3′ sequencing primer: CACTGCATTCTAGTTGTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry