Skip to main content
Addgene

OR2-consensus
(Plasmid #208060)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Total vector size (bp) 5014
  • Modifications to backbone
    Rhodopsin tag at N terminus
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OR2-consensus
  • Alt name
    consOR2
  • Species
    Synthetic
  • Insert Size (bp)
    1008
  • Promoter CMV
  • Tag / Fusion Protein
    • Rhodopsin tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTCCACAGGTGTCCACTC
  • 3′ sequencing primer CACTGCATTCTAGTTGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OR2-consensus was a gift from Hiroaki Matsunami (Addgene plasmid # 208060 ; http://n2t.net/addgene:208060 ; RRID:Addgene_208060)

  • For your References section:

    Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors. Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H. Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 10.1073/pnas.1915520117. Epub 2020 Jan 23. 10.1073/pnas.1915520117 PubMed 31974307
Related items: