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Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST
(Plasmid #208039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Derived from Addgene ID# 52962 (Zhang Lab).
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PML (isoform IVa; 3MAS)
  • Species
    H. sapiens (human)
  • Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
  • Entrez Gene
    PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
  • Promoter EFS
  • Tag / Fusion Protein
    • FLAG, N-terminal Split-TurboID (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TurboID fragments derived from Addgene ID # 107169 (Ting Lab). Lenti-EFS/P2A-blast backbone derived from Addgene ID# 52962 (Zhang Lab).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Control for PMLiva SUMO-ID experiments; 3MAS mutant lacks major SUMOylation sites and SIM domain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 208039 ; http://n2t.net/addgene:208039 ; RRID:Addgene_208039)

  • For your References section:

    Identification of proximal SUMO-dependent interactors using SUMO-ID. Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD. Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6. 10.1038/s41467-021-26807-6 PubMed 34795231
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