Ac5-STABLE2-blast
(Plasmid #208034)

• Purpose: (Empty Backbone) Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.
• Depositing Labs: Rosa Barrio, James Sutherland
• Publication: Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep00075. Epub 2011 Aug 31.

Backbone

• Vector backbone: Ac5/V5-HIS
• Backbone manufacturer: Invitrogen
• Vector type: Insect Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For multicistronic expression in insect cells. FLAG-mCherry, GFP, or Blast can be removed or replaced (swapped Nhe1-Xho1 NeoR from Addgene ID: 32426 for BlastR). N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible. Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.