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Addgene

Ac5-STABLE2-puro
(Plasmid #208032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Ac5/V5-HIS
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For multicistronic expression in insect cells. FLAG-mCherry, GFP, or Puro can be removed or replaced. (swapped Nhe1-Xho1 NeoR from Addgene ID: 32426 for PuroR). N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible. Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ac5-STABLE2-puro was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 208032 ; http://n2t.net/addgene:208032 ; RRID:Addgene_208032)

  • For your References section:

    Generation of stable Drosophila cell lines using multicistronic vectors. Gonzalez M, Martin-Ruiz I, Jimenez S, Pirone L, Barrio R, Sutherland JD. Sci Rep. 2011;1:75. doi: 10.1038/srep00075. Epub 2011 Aug 31. 10.1038/srep00075 PubMed 22355594
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