Skip to main content
Addgene

pCas3-Apr
(Plasmid #208000)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHERD30T
  • Backbone manufacturer
    Dongru Qiu, Hongwei D. Yu
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 11228
  • Modifications to backbone
    change of resistance cassette
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RhaR
  • Insert Size (bp)
    849
  • Entrez Gene
    rhaR (a.k.a. b3906, ECK3899, rhaC1)

Gene/Insert 2

  • Gene/Insert name
    RhaS
  • Insert Size (bp)
    837
  • Entrez Gene
    rhaS (a.k.a. b3905, ECK3898, rhaC2)

Gene/Insert 3

  • Gene/Insert name
    pRhaBAD
  • Insert Size (bp)
    36

Gene/Insert 4

  • Gene/Insert name
    I-C crRNA
  • Insert Size (bp)
    88

Gene/Insert 5

  • Gene/Insert name
    Cas3
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    2235

Gene/Insert 6

  • Gene/Insert name
    Cas5
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    654

Gene/Insert 7

  • Gene/Insert name
    Cas8
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    1764

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas3-Apr was a gift from Pablo Ivan Nikel (Addgene plasmid # 208000 ; http://n2t.net/addgene:208000 ; RRID:Addgene_208000)

  • For your References section:

    A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for Pseudomonas with efficient vector curing. Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, Hendrix H. Microbiol Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23. 10.1128/spectrum.02707-23 PubMed 37975669
Related items: