pCas3-Km
(Plasmid #207999)

• Purpose: Expresses all necessary components of Type I-C CRISPR-Cas system
• Depositing Lab: Pablo Ivan Nikel
• Publication: Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23.

Backbone

• Vector backbone: pHERD30T
• Backbone manufacturer: Dongru Qiu, Hongwei D. Yu
• Backbone size w/o insert (bp): 5216
• Total vector size (bp): 11176
• Modifications to backbone: change of resistance cassette
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RhaR
• Insert Size (bp): 849
• Entrez Gene: rhaR (a.k.a. b3906, ECK3899, rhaC1)

Gene/Insert 2

• Gene/Insert name: RhaS
• Insert Size (bp): 837
• Entrez Gene: rhaS (a.k.a. b3905, ECK3898, rhaC2)

Gene/Insert 3

• Gene/Insert name: pRhaBAD
• Insert Size (bp): 36

Gene/Insert 4

• Gene/Insert name: I-C crRNA
• Insert Size (bp): 88

Gene/Insert 5

• Gene/Insert name: Cas3
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 2235

Gene/Insert 6

• Gene/Insert name: Cas5
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 654

Gene/Insert 7

• Gene/Insert name: Cas8
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 1764

Resource Information

• Supplemental Documents:
  • pcas3-km.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.