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Addgene

pAf-CRISPR-ctfR1
(Plasmid #207992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPTRII
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 10031
  • Total vector size (bp) 13733
  • Modifications to backbone
    Removal of non-functional sequence of AMA1 in pPTRII, expression of cas9 using Aspergillus nidulans gpdA promoter and trpC terminator, expression of sgRNA using Aspergillus flavus U6 promoter and terminator
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ctfR1
  • gRNA/shRNA sequence
    GCTCTGAGCTGGCATTCCGC
  • Species
    Aspergillus flavus
  • Promoter Aspergillus flavus U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer ATACTGCAGTTCTCTTTAGAATTCAACTGTGGGT
  • 3′ sequencing primer TATGGTACCACATATTTAAAAAAAGTCTCCTGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAf-CRISPR-ctfR1 was a gift from Perng-Kuang Chang (Addgene plasmid # 207992 ; http://n2t.net/addgene:207992 ; RRID:Addgene_207992)

  • For your References section:

    Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B. Chang PK. Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. 10.1016/j.fgb.2023.103863 PubMed 38154756
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