pAAV-CaMKIIa-HA-eLACCO2.1-NGR
(Plasmid
#207989)
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PurposeBiosensor for extracellular L-lactate
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeLACCO2.1
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SpeciesSynthetic
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Insert Size (bp)2016
- Promoter CaMKII
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.12.27.522013 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CaMKIIa-HA-eLACCO2.1-NGR was a gift from Robert Campbell & Yuki Kambe (Addgene plasmid # 207989 ; http://n2t.net/addgene:207989 ; RRID:Addgene_207989) -
For your References section:
Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. Nasu Y, Aggarwal A, Le GNT, Vo CT, Kambe Y, Wang X, Beinlich FRM, Lee AB, Ram TR, Wang F, Gorzo KA, Kamijo Y, Boisvert M, Nishinami S, Kawamura G, Ozawa T, Toda H, Gordon GR, Ge S, Hirase H, Nedergaard M, Paquet ME, Drobizhev M, Podgorski K, Campbell RE. Nat Commun. 2023 Oct 27;14(1):6598. doi: 10.1038/s41467-023-42230-5. 10.1038/s41467-023-42230-5 PubMed 37891202
Map uploaded by the depositor.
