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Addgene

pENTR2b-mScarlet
(Plasmid #207961)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207961 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR2B
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 3755
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet
  • Species
    Synthetic
  • Insert Size (bp)
    696
  • GenBank ID
    KY021423.1
  • Promoter None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer attL1_F
  • 3′ sequencing primer attL2_R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR2b-mScarlet was a gift from Johanna Ivaska (Addgene plasmid # 207961 ; http://n2t.net/addgene:207961 ; RRID:Addgene_207961)

  • For your References section:

    Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling. Conway JRW, Isomursu A, Follain G, Harma V, Jou-Olle E, Pasquier N, Valimaki EPO, Rantala JK, Ivaska J. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2304288120. doi: 10.1073/pnas.2304288120. Epub 2023 Oct 16. 10.1073/pnas.2304288120 PubMed 37844244
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