pEF.DEST51-mScarlet
(Plasmid
#207960)
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PurposeVector for overexpression of the mScarlet fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF-DEST51
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Backbone manufacturerThermoFisher Scientific
- Backbone size w/o insert (bp) 7464
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet
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SpeciesSynthetic
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Insert Size (bp)696
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GenBank IDKY021423.1
- Promoter EF1a
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a_F
- 3′ sequencing primer BGH_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF.DEST51-mScarlet was a gift from Johanna Ivaska (Addgene plasmid # 207960 ; http://n2t.net/addgene:207960 ; RRID:Addgene_207960) -
For your References section:
Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling. Conway JRW, Isomursu A, Follain G, Harma V, Jou-Olle E, Pasquier N, Valimaki EPO, Rantala JK, Ivaska J. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2304288120. doi: 10.1073/pnas.2304288120. Epub 2023 Oct 16. 10.1073/pnas.2304288120 PubMed 37844244