pDEST-mCherry-ARHGEF2
(Plasmid #207959)

• Purpose: Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag
• Depositing Lab: Johanna Ivaska
• Publication: Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2304288120. doi: 10.1073/pnas.2304288120. Epub 2023 Oct 16.

Backbone

• Vector backbone: pDEST-N-term_mCherry
• Backbone manufacturer: A kind gift from Maria Vartiainen, University of Helsinki, Helsinki, Finland
• Backbone size w/o insert (bp): 7839
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARHGEF2
• Alt name: GEF-H1
• Species: H. sapiens (human)
• Insert Size (bp): 2879
• GenBank ID: 9181
• Entrez Gene: ARHGEF2 (a.k.a. GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: SV40pA_R

Resource Information

• Supplemental Documents:
  • pDEST-mCherry-ARHGEF2.gb
• A portion of this plasmid was derived from a plasmid made by: The entry clone pENTR221-ARHGEF2 was from the ORFeome library at the Genome Biology Unit core facility, supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland. This was used for the LR reaction with the pDEST-N-term_mCherry to generate the submitted plasmid, pDEST-mCherry-ARHGEF2.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry