TurboID-MCS MSCV-IRES-GFP
(Plasmid
#207958)
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PurposeRetroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV-IRES-GFP
- Backbone size w/o insert (bp) 6441
- Total vector size (bp) 7487
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Vector typeMammalian Expression, Retroviral ; TurboID biotin proximity ligase
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTurboID
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SpeciesSynthetic
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Tag
/ Fusion Protein
- TurboID (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TurboID-MCS MSCV-IRES-GFP was a gift from Timothy Ley (Addgene plasmid # 207958 ; http://n2t.net/addgene:207958 ; RRID:Addgene_207958) -
For your References section:
Proteogenomic analysis reveals cytoplasmic sequestration of RUNX1 by the acute myeloid leukemia-initiating CBFB::MYH11 oncofusion protein. Day RB, Hickman JA, Xu Z, Katerndahl CD, Ferraro F, Ramakrishnan SM, Erdmann-Gilmore P, Sprung RW, Mi Y, Townsend RR, Miller CA, Ley TJ. J Clin Invest. 2023 Dec 7:e176311. doi: 10.1172/JCI176311. 10.1172/JCI176311 PubMed 38061017
Map uploaded by the depositor.
