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pEndo-I-OnuI-TSM
(Plasmid #207954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC177
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 2953
  • Total vector size (bp) 5233
  • Modifications to backbone
    Inserted the I-OnuI TSM under the control of the BBa_J23108 promoter.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
  • Alt name
    I-OnuI TSM
  • Species
    S. cerevisiae (budding yeast); O. novo-ulmi
  • Insert Size (bp)
    2280
  • Mutation
    Leucine 212 changed to proline, leucine 434 in full-length fusion.

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEndo-I-OnuI-TSM was a gift from David Edgell (Addgene plasmid # 207954 ; http://n2t.net/addgene:207954 ; RRID:Addgene_207954)

  • For your References section:

    Intein-based thermoregulated meganucleases for containment of genetic material. Foo GW, Leichthammer CD, Saita IM, Lukas ND, Batko IZ, Heinrichs DE, Edgell DR. Nucleic Acids Res. 2024 Feb 28;52(4):2066-2077. doi: 10.1093/nar/gkad1247. 10.1093/nar/gkad1247 PubMed 38180814
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