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Addgene

pKE014
(Plasmid #207908)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9_introns_2xNLS-Pea3A(T) term
  • Species
    Other

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKE014 was a gift from Alexis Maizel (Addgene plasmid # 207908 ; http://n2t.net/addgene:207908 ; RRID:Addgene_207908)
  • For your References section:

    GreenGate 2.0: Backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate. Piepers M, Erbstein K, Reyes-Hernandez J, Song C, Tessi T, Petrasiunaite V, Faerber N, Distel K, Maizel A. PLoS One. 2023 Sep 8;18(9):e0290097. doi: 10.1371/journal.pone.0290097. eCollection 2023. 10.1371/journal.pone.0290097 PubMed 37682951