VHL-pGex2TK
(Plasmid #20790)

• Depositing Lab: William Kaelin
• Publication: Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Backbone

• Vector backbone: pGex2TK
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4959
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: - DH5alpha for propagation - BL21 or similar for expression
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VHL
• Species: H. sapiens (human)
• Insert Size (bp): 652
• Mutation: VHL wt cDNA, no mutations
• GenBank ID: NM_000551
• Entrez Gene: VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: pGex-5'
• 3′ sequencing primer: pGex-3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry