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pTarget-Dual
(Plasmid #207884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 3699
  • Total vector size (bp) 3743
  • Modifications to backbone
    CmR swapped for KanR, p15A ori swapped for pBR322, inserted the I-OnuI and I-GpeMI target sites, Cen6-ArsH4-His3 included for yeast assemblies.
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    I-OnuI target site
  • Species
    O. novo-ulmi
  • Insert Size (bp)
    22

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    I-GpeMI target site
  • Species
    G. penicillata
  • Insert Size (bp)
    22

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Edgell Lab uses EPI300 E. coli for transformation, but DH5alpha will also be fine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTarget-Dual was a gift from David Edgell (Addgene plasmid # 207884 ; http://n2t.net/addgene:207884 ; RRID:Addgene_207884)

  • For your References section:

    Intein-based thermoregulated meganucleases for containment of genetic material. Foo GW, Leichthammer CD, Saita IM, Lukas ND, Batko IZ, Heinrichs DE, Edgell DR. Nucleic Acids Res. 2024 Feb 28;52(4):2066-2077. doi: 10.1093/nar/gkad1247. 10.1093/nar/gkad1247 PubMed 38180814
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