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Addgene

pRset-rogRFP2
(Plasmid #207875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRset
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2764
  • Total vector size (bp) 4249
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rogRFP2
  • Alt name
    roGFP2-mApple
  • Species
    Synthetic
  • Insert Size (bp)
    1484
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRset-rogRFP2 was a gift from Mathew Tantama (Addgene plasmid # 207875 ; http://n2t.net/addgene:207875 ; RRID:Addgene_207875)

  • For your References section:

    Neuron Activity Dependent Redox Compartmentation Revealed with a Second Generation Red-Shifted Ratiometric Sensor. Radhakrishnan S, Norley J, Wendt S, LeRoy N, Hall H, Norcross S, Doan S, Snaider J, MacVicar BA, Weake VM, Huang L, Tantama M. ACS Chem Neurosci. 2020 Sep 2;11(17):2666-2678. doi: 10.1021/acschemneuro.0c00342. Epub 2020 Aug 21. 10.1021/acschemneuro.0c00342 PubMed 32786310
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