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Addgene

GW1-mScarlet-Epac-GeNL
(Plasmid #207874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GW1
  • Backbone size w/o insert (bp) 4461
  • Total vector size (bp) 9362
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
  • Alt name
    mScarlet-hEpac1(149-881)-GeNL
  • Species
    Synthetic
  • Insert Size (bp)
    4092
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGAATCGGAGTGAGTGC
  • 3′ sequencing primer CAAATGTGGTATGGCTGATT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GW1-mScarlet-Epac-GeNL was a gift from Mathew Tantama (Addgene plasmid # 207874 ; http://n2t.net/addgene:207874 ; RRID:Addgene_207874)

  • For your References section:

    Dual-Mode FRET and BRET Sensors for Detecting cAMP Dynamics. French AR, Tesmer AL, Tantama M. ACS Omega. 2019 Sep 12;4(13):15504-15511. doi: 10.1021/acsomega.9b01770. eCollection 2019 Sep 24. 10.1021/acsomega.9b01770 PubMed 31572851
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