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pCMV5 Flag p38 alpha(agf)
(Plasmid #20787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4657
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCMV5 Flag p38 alpha (agf)
  • Alt name
    MapK14
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1127
  • Mutation
    replaced Thr180 and Tyr182 with Ala and Phe, respectively
  • GenBank ID
    NM_011951
  • Entrez Gene
    Mapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer CMV F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original wild-type p38 alpha insert provided by Jiahuai Han and Richard J. Ulevitch, "A Map Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells", (Science,vol.265, pg806)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag p38 alpha(agf) was a gift from Roger Davis (Addgene plasmid # 20787 ; http://n2t.net/addgene:20787 ; RRID:Addgene_20787)

  • For your References section:

    Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ, Davis RJ. J Biol Chem. 1995 Mar 31. 270(13):7420-6. 10.1074/jbc.270.13.7420 PubMed 7535770
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