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Addgene

GW1-mCherryEA
(Plasmid #207867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GW1
  • Backbone size w/o insert (bp) 5274
  • Total vector size (bp) 5984
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherryEA
  • Alt name
    mCherry(I158E/Q160A)
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGAATCGGAGTGAGTGC
  • 3′ sequencing primer CAAATGTGGTATGGCTGATT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GW1-mCherryEA was a gift from Mathew Tantama (Addgene plasmid # 207867 ; http://n2t.net/addgene:207867 ; RRID:Addgene_207867)

  • For your References section:

    Imaging pH Dynamics Simultaneously in Two Cellular Compartments Using a Ratiometric pH-Sensitive Mutant of mCherry. Rajendran M, Claywell B, Haynes EP, Scales U, Henning CK, Tantama M. ACS Omega. 2018 Aug 31;3(8):9476-9486. doi: 10.1021/acsomega.8b00655. Epub 2018 Aug 20. 10.1021/acsomega.8b00655 PubMed 30197999
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