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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
(Plasmid #207863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p601 modified
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
  • Species
    Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    2856
  • Mutation
    See Manuscript
  • Promoter Cbh

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCGAGTCAGTGAGCGAG
  • 3′ sequencing primer GTTTTCCCAGTCACGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP was a gift from David Liu (Addgene plasmid # 207863 ; http://n2t.net/addgene:207863 ; RRID:Addgene_207863)

  • For your References section:

    Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR. Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. 10.1016/j.cell.2023.07.039 PubMed 37657419
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