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Addgene

pCMV-PE6d
(Plasmid #207854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE6d
  • Alt name
    Cas9(R221K, N394K, H840A)-M-MLVRTDRNAseHrt(T128N, V223Y, D200C)
  • Species
    Synthetic
  • Insert Size (bp)
    5856
  • Mutation
    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)
  • Tags / Fusion Proteins
    • SV40 bpNLS (N terminal on insert)
    • SV40 bpNLS, c-Myc NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PE6d was a gift from David Liu (Addgene plasmid # 207854 ; http://n2t.net/addgene:207854 ; RRID:Addgene_207854)

  • For your References section:

    Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR. Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. 10.1016/j.cell.2023.07.039 PubMed 37657419
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