pCMV-PE6b
(Plasmid #207852)

• Purpose: Mammalian expression of PE6b prime editor
• Depositing Lab: David Liu
• Publication: Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039.

Backbone

• Vector backbone: pCMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE6b
• Alt name: Cas9(R221K, N394K, H840A)-Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128V, L158Q, F269L, A363V, K413E, S492N)
• Species: Synthetic
• Insert Size (bp): 5880
• Mutation: Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128V, L158Q, F269L, A363V, K413E, S492N)
• Tags / Fusion Proteins:
  • SV40 bpNLS (N terminal on insert)
  • SV40 bpNLS, c-Myc NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7-forward
• 3′ sequencing primer: BGH-reverse

Resource Information

• Supplemental Documents:
  • pCMV-PE6b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry