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PurposeMammalian expression of PE6a prime editor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE6a
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Alt nameCas9(R221K, N394K, H840A)-Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K318E, K343N)
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SpeciesSynthetic
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Insert Size (bp)5586
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MutationEc48rt(E60K, K87E, E165D, D243N, R267I, E279K, K318E, K343N)
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Tags
/ Fusion Proteins
- SV40 bpNLS (N terminal on insert)
- SV40 bpNLS, c-Myc NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-PE6a was a gift from David Liu (Addgene plasmid # 207851 ; http://n2t.net/addgene:207851 ; RRID:Addgene_207851) -
For your References section:
Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR. Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. 10.1016/j.cell.2023.07.039 PubMed 37657419