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CBla_2.0_EmrE_TMD4_3P_G90V_G97V
(Plasmid #207849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD322K (modified)
  • Backbone manufacturer
    John E. Cronan
  • Backbone size w/o insert (bp) 4284
  • Total vector size (bp) 5253
  • Modifications to backbone
    Deletion 4246–4454/0–2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    C-BLa 2.0 fusion protein
  • Species
    Synthetic
  • Insert Size (bp)
    969
  • Tag / Fusion Protein
    • Flag (DYKDHDG) (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TMD: Integrin alphaV mut GP, contains ApaI restriction site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBla_2.0_EmrE_TMD4_3P_G90V_G97V was a gift from Dieter Langosch (Addgene plasmid # 207849 ; http://n2t.net/addgene:207849 ; RRID:Addgene_207849)

  • For your References section:

    BLaTM 2.0, a genetic tool revealing preferred antiparallel interaction of transmembrane helix 4 of the dual-topology protein EmrE. Julius A, Laur L, Schanzenbach C, Langosch D. J Mol Biol. 2017 Apr 18. pii: S0022-2836(17)30166-3. doi: 10.1016/j.jmb.2017.04.003. 10.1016/j.jmb.2017.04.003 PubMed 28432015
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