CBla_2.0_EmrE_TMD4_3P
(Plasmid
#207848)
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PurposeBLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD322K (modified)
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Backbone manufacturerJohn E. Cronan
- Backbone size w/o insert (bp) 4284
- Total vector size (bp) 5253
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Modifications to backboneDeletion 4246–4454/0–2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameC-BLa 2.0 fusion protein
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SpeciesSynthetic
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Insert Size (bp)969
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Tag
/ Fusion Protein
- Flag (DYKDHDG) (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TMD: Integrin alphaV mut GP, contains ApaI restriction site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBla_2.0_EmrE_TMD4_3P was a gift from Dieter Langosch (Addgene plasmid # 207848 ; http://n2t.net/addgene:207848 ; RRID:Addgene_207848) -
For your References section:
BLaTM 2.0, a genetic tool revealing preferred antiparallel interaction of transmembrane helix 4 of the dual-topology protein EmrE. Julius A, Laur L, Schanzenbach C, Langosch D. J Mol Biol. 2017 Apr 18. pii: S0022-2836(17)30166-3. doi: 10.1016/j.jmb.2017.04.003. 10.1016/j.jmb.2017.04.003 PubMed 28432015