CBLa_1.2_GpA_wt
(Plasmid
#207843)
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PurposeBLaTM-System GpA wt positive control in CBLa 1.2 vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD322K (modified)
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Backbone manufacturerJohn E. Cronan
- Backbone size w/o insert (bp) 4232
- Total vector size (bp) 5522
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Modifications to backboneDeletion 4246–4454/0–2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameN-BLa 1.2 fusion protein
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SpeciesSynthetic
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Insert Size (bp)1527
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TMD: Integrin alphaV mut GP, contains ApaI restriction site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBLa_1.2_GpA_wt was a gift from Dieter Langosch (Addgene plasmid # 207843 ; http://n2t.net/addgene:207843 ; RRID:Addgene_207843) -
For your References section:
Identifying ionic interactions within a membrane using BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Schanzenbach C, Schmidt FC, Breckner P, Teese MG, Langosch D. Sci Rep. 2017 Mar 7;7:43476. doi: 10.1038/srep43476. 10.1038/srep43476 PubMed 28266525