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PIEZO1-HaloTag
(Plasmid #207834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 12416
  • Modifications to backbone
    No GFP expression IRES removed
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIEZO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8463
  • Entrez Gene
    PIEZO1 (a.k.a. DHS, ER, FAM38A, LMPH3, LMPHM6, Mib)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original PIEZO1 clone from Philip Gottlieb, SUNY, Buffalo.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PIEZO1-HaloTag was a gift from Charles Cox (Addgene plasmid # 207834 ; http://n2t.net/addgene:207834 ; RRID:Addgene_207834)

  • For your References section:

    MyoD-family inhibitor proteins act as auxiliary subunits of Piezo channels. Zhou Z, Ma X, Lin Y, Cheng D, Bavi N, Secker GA, Li JV, Janbandhu V, Sutton DL, Scott HS, Yao M, Harvey RP, Harvey NL, Corry B, Zhang Y, Cox CD. Science. 2023 Aug 18;381(6659):799-804. doi: 10.1126/science.adh8190. Epub 2023 Aug 17. 10.1126/science.adh8190 PubMed 37590348
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