PIEZO1-HaloTag
(Plasmid #207834)

• Purpose: PIEZO1 fusion for biochemistry and live imaging
• Depositing Lab: Charles Cox
• Publication: Zhou et al Science. 2023 Aug 18;381(6659):799-804. doi: 10.1126/science.adh8190. Epub 2023 Aug 17.

Backbone

• Vector backbone: pIRES2
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 12416
• Modifications to backbone: No GFP expression IRES removed
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PIEZO1
• Species: H. sapiens (human)
• Insert Size (bp): 8463
• Entrez Gene: PIEZO1 (a.k.a. DHS, ER, FAM38A, LMPH3, LMPHM6, Mib)
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original PIEZO1 clone from Philip Gottlieb, SUNY, Buffalo.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry