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Addgene

pRS405-FlagPEN2/PS1wt
(Plasmid #207803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS405
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gamma-secretase subunits PEN-2 and presenilin1 wt
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a F201L mutation in presenilin-1 compared to the NCBI reference sequence NP_015557.2. This mutation is not know to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS405-FlagPEN2/PS1wt was a gift from Dieter Langosch (Addgene plasmid # 207803 ; http://n2t.net/addgene:207803 ; RRID:Addgene_207803)

  • For your References section:

    Permissive Conformations of a Transmembrane Helix Allow Intramembrane Proteolysis by gamma-Secretase. Ortner M, Guschtschin-Schmidt N, Stelzer W, Muhle-Goll C, Langosch D. J Mol Biol. 2023 Aug 1;435(18):168218. doi: 10.1016/j.jmb.2023.168218. 10.1016/j.jmb.2023.168218 PubMed 37536392
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