pX330-PITCh-TUBB4B
(Plasmid
#207785)
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PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepX330-BbsI-PITCh
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Backbone manufacturerAddgene #127875
- Backbone size w/o insert (bp) 8947
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesgRNA Targeting C-terminus of TUBB4B
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Alt nameTUBB4B
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gRNA/shRNA sequenceCCTAGAGCCTTCAGTCACTG
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SpeciesH. sapiens (human)
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Insert Size (bp)20
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Entrez GeneTUBB4B (a.k.a. Beta2, LCAEOD, TUBB2, TUBB2C)
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCCTTTTTACGGTTCCTGGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX330-PITCh-TUBB4B was a gift from Laurence Pelletier (Addgene plasmid # 207785 ; http://n2t.net/addgene:207785 ; RRID:Addgene_207785) -
For your References section:
qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes. Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L. bioRxiv 10.1101/2023.11.01.565029
