pX330-PITCh-H3C2
(Plasmid #207780)

• Purpose: Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.
• Depositing Lab: Laurence Pelletier
• Publication: Philip et al bioRxiv

Backbone

• Vector backbone: pX330-BbsI-PITCh
• Backbone manufacturer: Addgene #127875
• Backbone size w/o insert (bp): 8947
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sgRNA Targeting C-terminus of H3C2
• Alt name: H3C2
• gRNA/shRNA sequence: ACGCTCTTTCTCCGCGAATG
• Species: H. sapiens (human)
• Insert Size (bp): 20
• Entrez Gene: H3C2 (a.k.a. H3/l, H3C1, H3C10, H3C11, H3C12, H3C3, H3C4, H3C6, H3C7, H3C8, H3FL, HIST1H3B)
• Promoter: U6

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GCCTTTTTACGGTTCCTGGC

Resource Information

• Supplemental Documents:
  • pX330-PITCh-H3C2.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry