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Addgene

pEF1α-Cre-PGK-Puro
(Plasmid #207742)

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Item Catalog # Description Quantity Price (USD)
Plasmid 207742 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre-PGK-Puro
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    3740
  • Promoter EF1alpha

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGCAAGGCGATTAAGTTGGGTAAC
  • 3′ sequencing primer GGCTCGTATGTTGTGTGGAATTGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1α-Cre-PGK-Puro was a gift from Laurence Pelletier (Addgene plasmid # 207742 ; http://n2t.net/addgene:207742 ; RRID:Addgene_207742)

  • For your References section:

    qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes. Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L. bioRxiv 10.1101/2023.11.01.565029
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