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pACYC Destination
(Plasmid #207675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC
  • Modifications to backbone
    pACYC backbone harbored an operon for constitutive expression of the repressors for 3 inducible promoters (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter CymRC, PhlF, VanCC

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    suggested Chloramphenicol concentration: 34 µg/mL
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Marionette Sensor Collection was a gift from Christopher Voigt (Addgene Kit #1000000137).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYC Destination was a gift from Ramon Gonzalez (Addgene plasmid # 207675 ; http://n2t.net/addgene:207675 ; RRID:Addgene_207675)

  • For your References section:

    Metabolic flux optimization of iterative pathways through orthogonal gene expression control: Application to the beta-oxidation reversal. Lee SH, Hu Y, Chou A, Chen J, Gonzalez R. Metab Eng. 2024 Mar;82:262-273. doi: 10.1016/j.ymben.2024.02.007. Epub 2024 Feb 20. 10.1016/j.ymben.2024.02.007 PubMed 38387675
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