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Addgene

pDACR3882 [rab-3p::HYlight::let-858utr]
(Plasmid #207666)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207666 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST[R4-R3]
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2445
  • Total vector size (bp) 5925
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HYlight Biosensor
  • Alt name
    CggR-cpGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1569
  • Promoter rab-3

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://pubmed.ncbi.nlm.nih.gov/37662365/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDACR3882 [rab-3p::HYlight::let-858utr] was a gift from Daniel Colón-Ramos (Addgene plasmid # 207666 ; http://n2t.net/addgene:207666 ; RRID:Addgene_207666)
  • For your References section:

    Local and dynamic regulation of neuronal glycolysis in vivo. Wolfe AD, Koberstein JN, Smith CB, Stewart ML, Gonzalez IJ, Hammarlund M, Hyman AA, Stork PJS, Goodman RH, Colon-Ramos DA. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. Epub 2024 Jan 10. 10.1073/pnas.2314699121 PubMed 38198527