pDACR3882 [rab-3p::HYlight::let-858utr]
(Plasmid
#207666)
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PurposeExpresses worm-optimized HYlight in C. elegans neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST[R4-R3]
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2445
- Total vector size (bp) 5925
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHYlight Biosensor
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Alt nameCggR-cpGFP
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SpeciesSynthetic
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Insert Size (bp)1569
- Promoter rab-3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://pubmed.ncbi.nlm.nih.gov/37662365/ for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDACR3882 [rab-3p::HYlight::let-858utr] was a gift from Daniel Colón-Ramos (Addgene plasmid # 207666 ; http://n2t.net/addgene:207666 ; RRID:Addgene_207666) -
For your References section:
Local and dynamic regulation of neuronal glycolysis in vivo. Wolfe AD, Koberstein JN, Smith CB, Stewart ML, Gonzalez IJ, Hammarlund M, Hyman AA, Stork PJS, Goodman RH, Colon-Ramos DA. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. Epub 2024 Jan 10. 10.1073/pnas.2314699121 PubMed 38198527