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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
(Plasmid #207664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3
  • Backbone size w/o insert (bp) 5116
  • Total vector size (bp) 8069
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEP
  • Alt name
    Neprilysin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2953
  • Mutation
    Addition of signaling peptide (POMC) on the N-terminus
  • GenBank ID
    NP_000893.2
  • Entrez Gene
    MME (a.k.a. CALLA, CD10, CMT2T, NEP, SCA43, SFE)
  • Promoter hSynapsin
  • Tag / Fusion Protein
    • mRUBY3 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ACTTGCAAATGGGCTTGCCACGCACTTC
  • 3′ sequencing primer CTAGAATTCGGTACCGCTAGCCCCCACCATGCCGAGATC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.01.19.524797 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE was a gift from Sung Han (Addgene plasmid # 207664 ; http://n2t.net/addgene:207664 ; RRID:Addgene_207664)

  • For your References section:

    Novel genetically encoded tools for imaging or silencing neuropeptide release from presynaptic terminals in vivo. Kim D-I, Park S, Ye M, Chen JY, Jhang J, Hunker AC, Zweifel LS, Palmiter RD, Han S. bioRxiv 2023.01.19.524797 10.1101/2023.01.19.524797
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