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Addgene

pEVOL-NBK-1
(Plasmid #207639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
  • Species
    Synthetic
  • Mutation
    The evolved synthetase NBK-1 contains mutations Y306M, L309A, C348A, Y384F.
  • Promoter araBAD

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-NBK-1 was a gift from Cole DeForest (Addgene plasmid # 207639 ; http://n2t.net/addgene:207639 ; RRID:Addgene_207639)

  • For your References section:

    Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation. Ruskowitz ER, Munoz-Robles BG, Strange AC, Butcher CH, Kurniawan S, Filteau JR, DeForest CA. Nat Chem. 2023 May;15(5):694-704. doi: 10.1038/s41557-023-01152-x. Epub 2023 Apr 17. 10.1038/s41557-023-01152-x PubMed 37069270
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