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Addgene

LgBiT-ST
(Plasmid #207633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29
  • Backbone size w/o insert (bp) 5211
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LgBiT-SpyTag
  • Species
    Synthetic
  • Insert Size (bp)
    573
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LgBiT-ST was a gift from Cole DeForest (Addgene plasmid # 207633 ; http://n2t.net/addgene:207633 ; RRID:Addgene_207633)

  • For your References section:

    Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation. Ruskowitz ER, Munoz-Robles BG, Strange AC, Butcher CH, Kurniawan S, Filteau JR, DeForest CA. Nat Chem. 2023 May;15(5):694-704. doi: 10.1038/s41557-023-01152-x. Epub 2023 Apr 17. 10.1038/s41557-023-01152-x PubMed 37069270
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