pCMV-LambdaN-NbALFA
(Plasmid
#207627)
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PurposeExpresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepiggyBAC-transposon
- Total vector size (bp) 5726
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLambdaN-NbALFA
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SpeciesSynthetic
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Insert Size (bp)540
- Promoter pCMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.08.14.553235 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-LambdaN-NbALFA was a gift from Jay Shendure (Addgene plasmid # 207627 ; http://n2t.net/addgene:207627 ; RRID:Addgene_207627) -
For your References section:
A molecular proximity sensor based on an engineered, dual-component guide RNA. Choi J, Chen W, Liao H, Li X, Shendure J. eLife 2024 [Reviewed Preprint] 13:RP98110 10.7554/eLife.98110.2
