IL1_1
(Plasmid
#207623)
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PurposeLow copy plasmid expressing mTurquoise2 under the TDH3p promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMoClo-YTK
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5795
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemTurquoise2
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SpeciesAequorea victoria
- Promoter ScTDH3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Golden Gate Assembly (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMoClo-YTK kit
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IL1_1 was a gift from Yvonne Nygård (Addgene plasmid # 207623 ; http://n2t.net/addgene:207623 ; RRID:Addgene_207623) -
For your References section:
Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library. Mormino M, Lenitz I, Siewers V, Nygard Y. Microb Cell Fact. 2022 Oct 15;21(1):214. doi: 10.1186/s12934-022-01938-7. 10.1186/s12934-022-01938-7 PubMed 36243715