pCS2-Opto-Alk3
(Plasmid
#207614)
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PurposeZebrafish Alk3 BMP receptor kinase domain fused to VfLOV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 5760
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAlk3 (bmpr1aa) kinase domain + VfLOV domain
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SpeciesD. rerio (zebrafish); Vaucheria frigida
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Entrez Genebmpr1aa (a.k.a. BMPR-IA, BR1a, alk3, alk3tr, bmpr1a, zgc:136731)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-Opto-Alk3 was a gift from Patrick Müller (Addgene plasmid # 207614 ; http://n2t.net/addgene:207614 ; RRID:Addgene_207614) -
For your References section:
Optogenetic investigation of BMP target gene expression diversity. Rogers KW, ElGamacy M, Jordan BM, Muller P. Elife. 2020 Nov 11;9:e58641. doi: 10.7554/eLife.58641. 10.7554/eLife.58641 PubMed 33174840