vDip-C: pAAV-Pcp2-mGreenLantern-KASH
(Plasmid
#207613)
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PurposevDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 5893
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGreenLantern
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SpeciesSynthetic
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Insert Size (bp)717
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GenBank ID
- Promoter mouse Pcp2 promoter (L7-6)
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Tag
/ Fusion Protein
- KASH (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
vDip-C: pAAV-Pcp2-mGreenLantern-KASH was a gift from Longzhi Tan (Addgene plasmid # 207613 ; http://n2t.net/addgene:207613 ; RRID:Addgene_207613) -
For your References section:
Lifelong restructuring of 3D genome architecture in cerebellar granule cells. Tan L, Shi J, Moghadami S, Parasar B, Wright CP, Seo Y, Vallejo K, Cobos I, Duncan L, Chen R, Deisseroth K. Science. 2023 Sep 8;381(6662):1112-1119. doi: 10.1126/science.adh3253. Epub 2023 Sep 7. 10.1126/science.adh3253 PubMed 37676945