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Addgene

pDWJ27
(Plasmid #207604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-BIB
  • Species
    E. coli
  • Insert Size (bp)
    1539

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene was synthesised with codon optimisation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDWJ27 was a gift from Colin Kleanthous (Addgene plasmid # 207604 ; http://n2t.net/addgene:207604 ; RRID:Addgene_207604)

  • For your References section:

    Tunable force transduction through the Escherichia coli cell envelope. Williams-Jones DP, Webby MN, Press CE, Gradon JM, Armstrong SR, Szczepaniak J, Kleanthous C. Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2306707120. doi: 10.1073/pnas.2306707120. Epub 2023 Nov 16. 10.1073/pnas.2306707120 PubMed 37972066
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