pCasso
(Plasmid
#207530)
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PurposeConditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA448
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Backbone manufacturerSEVA collection
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Vector typeCRISPR ; Pseudomonas vector
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCasso was a gift from Pablo Ivan Nikel (Addgene plasmid # 207530 ; http://n2t.net/addgene:207530 ; RRID:Addgene_207530) -
For your References section:
The pAblo.pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria. Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI. Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236. 10.1093/nar/gkad1236 PubMed 38180826