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Addgene

pMCRi
(Plasmid #207524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207524 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA448
  • Vector type
    Pseudomonas vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCRi was a gift from Pablo Ivan Nikel (Addgene plasmid # 207524 ; http://n2t.net/addgene:207524 ; RRID:Addgene_207524)

  • For your References section:

    The pAblo.pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria. Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI. Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236. 10.1093/nar/gkad1236 PubMed 38180826
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