pLenti_EF1a_TauFRET_3xKQ_wpre
(Plasmid
#207522)
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PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneVectorBuilder pLV
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 7300
- Total vector size (bp) 9700
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTauFRET_P301L_3xKQ
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SpeciesH. sapiens (human)
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Insert Size (bp)2380
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MutationP301L; K311Q; K317Q; K321Q
- Promoter EF1a
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Tags
/ Fusion Proteins
- mTurquoise2
- NeonGreen
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_EF1a_TauFRET_3xKQ_wpre was a gift from Bradley Hyman (Addgene plasmid # 207522 ; http://n2t.net/addgene:207522 ; RRID:Addgene_207522) -
For your References section:
Specific detection of tau seeding activity in Alzheimer's disease using rationally designed biosensor cells. Lathuiliere A, Jo Y, Perbet R, Donahue C, Commins C, Quittot N, Fan Z, Bennett RE, Hyman BT. Mol Neurodegener. 2023 Aug 8;18(1):53. doi: 10.1186/s13024-023-00643-2. 10.1186/s13024-023-00643-2 PubMed 37553663
